Separation of choline from Coptidis Rhizoma and impact on glucose metabolism of berberine in HepG2 cells / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1771-1774, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-338764
ABSTRACT
<p><b>OBJECTIVE</b>To investigate action of hydrophilic constituents from Coptidis Rhizoma on glucose-lowering effect metabolism of berberine in HepG2 cells.</p><p><b>METHOD</b>Hydrophilic fractions of Coptidis Rhizoma were prepared by high speed counter current chromatography and separated by silica gel column chromatography. MTT assay was used to monitor the proliferation of HepG2 cells, and Kit was used to test the glucose consumption in culture solution.</p><p><b>RESULT</b>Choline was separated from Coptidis Rhizoma for the first time. Cell assay showed choline can significantly increase the glucose lowering effect of berberine and improve the cytotoxicity of berberine within test concentration. Compared with same dose of berberine, berberine 38 mg x L(-1) in combination with choline 100 mg x L(-1) can make glucose consumption increase by 34% and elevate cell livability up to 75% in HepG2 cells.</p><p><b>CONCLUSION</b>The results suggest that choline had a synergistic effect on improving glucose absorption of berberine and decreasing cytotoxicity of berberine.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Berberina
/
Medicamentos de Ervas Chinesas
/
Química
/
Colina
/
Cromatografia Líquida de Alta Pressão
/
Proliferação de Células
/
Sinergismo Farmacológico
/
Absorção
/
Células Hep G2
Limite:
Humanos
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2012
Tipo de documento:
Artigo
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