MTT assay for detecting 5-fluorouracil chemosensitivity of human breast carcinoma cell line / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To assess the feasibility of MTT colorimetric assay for testing the in vitro chemosensitivity of breast cancer cells to 5 fluorouracil (5-Fu).</p><p><b>METHODS</b>The chemosensitivity of human breast carcinoma cell lines MCF-7 and MDA-MB-435S to 5-Fu at different concentrations was evaluated with MTT assay.</p><p><b>RESULTS</b>5-FU treatment resulted in dose-dependent growth inhibition of the breast cancer cells with both low and high metastatic capacities.</p><p><b>CONCLUSIONS</b>MTT assay may help select appropriate chemotherapeutic agents and provides evidence for individualized chemotherapy for breast cancer.</p>