Effect of recombinant caspase-3 gene transfection on CNE2 cell apoptosis / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the apoptosis-inducing effect of caspase-3 on human nasopharyngeal carcinoma cells (CNE2).</p><p><b>METHODS</b>Recombinant caspase-3 was subcloned into the eukaryotic expression vector PEGFP-C1 containing the reporter gene using DNA recombinant technique. CNE2 cells were transfected with the recombinant caspase-3 gene via lipofectamine 2000 and the expression of caspase-3 mRNA was detected by reverse transcription-polymerase chain reaction. The cell morphological changes were observed under fluorescence microscope and electron microscope and the cell survival rate after the transfection was assessed by MTT assay.</p><p><b>RESULTS</b>Transfection with the recombinant caspase-3 gene induced significant apoptosis in CNE2 cells, which exhibited obvious morphological changes typical of apoptotic cells.</p><p><b>CONCLUSION</b>The recombinant caspase-3 gene can inhibit the growth and effectively induce apoptosis of CNE2 cells.</p>