Therapeutic effect of secretive endostatin eukaryotic expressing plasmid on mouse hepatoma / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 716-718, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-339103
ABSTRACT
<p><b>OBJECTIVE</b>To construct and express secretive endostatin eukaryotic plasmid for treatment of hepatoma.</p><p><b>METHODS</b>Mouse Igk signal peptide sequence was synthesized and cloned into pcDNA3.1 with endostatin gene. The supernant of BHK-21 transfected with recombinant was used to culture ECV304. The proliferation of latter was evaluated by MTT assay. H22 was inoculated intramusclely, then naked DNA of endostatin plasmid was injected into the inoculation site. Tumors were dissected and weighted after treatments. All data was analyzed by SPSS10.0.</p><p><b>RESULTS</b>The supernant of BHK-21 transfected with recombinant can inhibit the proliferation of ECV304 by 29.2%. Tumor weight lighter after injected with naked pSecES (1.34 g+/-0.96g) compared with naked pcDNA3.1 (2.70g+/-0.82g) and saline (3.73g+/-1.41g).</p><p><b>CONCLUSION</b>The endostatin eukaryotic plasmid was constructed and it can be used for gene therapy on hepatoma.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmídeos
/
Terapêutica
/
Terapia Genética
/
Secreções Corporais
/
Endostatinas
/
Genética
/
Neoplasias Hepáticas Experimentais
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Hepatology
Ano de publicação:
2003
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS