Differentiation of bone marrow stem cells in rat hepatic fibrogenesis environment / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 673-675, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-339123
ABSTRACT
<p><b>OBJECTIVE</b>To observe the differentiation of bone marrow stem cells in rat hepatic fibrogenesis environment into hepatocytes.</p><p><b>METHODS</b>Rat hepatic fibrosis was induced by subcutaneous injection of CCl4. Bone marrow stem cells with Thy positive, CD3 and CD45RA negative were enriched from the bone marrow by fluorescence-activated cell sorting. The bone marrow stem cells were labeled with PKH26-GL, and then autotransplanted. After six weeks, albumin, ck8 and a-smooth muscle actin expression were determined by immunocytochemistry.</p><p><b>RESULTS</b>The PKH26-GL labeled cells expressed albumin and ck8, but did not express a-smooth muscle actin in hepatic fibrogenesis environment.</p><p><b>CONCLUSION</b>Bone marrow stem cells in hepatic fibrogenesis environment can differentiate into hepatocytes, but can't differentiate into myofibroblasts</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Fisiologia
/
Células da Medula Óssea
/
Células-Tronco Hematopoéticas
/
Diferenciação Celular
/
Ratos Sprague-Dawley
/
Hepatócitos
/
Meio Ambiente
/
Cirrose Hepática
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Hepatology
Ano de publicação:
2003
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS