Cloning and expressing the E2 subunit of pyruvate dehydrogenase complex / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 602-604, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-339154
ABSTRACT
<p><b>OBJECTIVES</b>To construct the expression vector of the pyruvate dehydrogenase complex E2 subunit gene (PDC-E2).</p><p><b>METHODS</b>The PDC-E2 gene was amplified from human lymphocytes with RT-PCR, and was cloned into pExSecI vector to induce the PDC-E2 expression. The products were identified with western blot and ELISA.</p><p><b>RESULTS</b>The expression vector pExSecI/PDC-E2 was successfully constructed. The products could be identified by the specific self-antibodies in the sera from the primary biliary cirrhosis patients.</p><p><b>CONCLUSION</b>High efficient expression vector of PDC-E2 lays the foundation for serum assay of primary biliary cirrhosis patients with prokaryotic expressing PDC-E2.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Complexo Piruvato Desidrogenase
/
Sangue
/
Ensaio de Imunoadsorção Enzimática
/
Linfócitos
/
Reação em Cadeia da Polimerase
/
Clonagem Molecular
/
Diagnóstico
/
Alergia e Imunologia
/
Di-Hidrolipoil-Lisina-Resíduo Acetiltransferase
/
Genética
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Hepatology
Ano de publicação:
2003
Tipo de documento:
Artigo
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