In vitro study of directional inducible differentiation of inner ear hair cells from human adipose-derived mesenchymal stem cells / 中华耳鼻咽喉头颈外科杂志
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
; (12): 323-328, 2009.
Article
em Zh
| WPRIM
| ID: wpr-339207
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the feasibility of directionally inducing human adipose-derived mesenchymal stem cells (hAD-MSCs) towards inner ear hair cells.</p><p><b>METHODS</b>Mesenchymal stem cells from human adipose tissue were isolated, purified and cultured in vitro. hAD-MSCs were induced to neural stem/progenitor-like cell, and then co-cultured with embryonic chick otic vesicle cells. Processed hAD-MSCs were tested by immunostaining to ascertain whether they expressed characteristic hair cell markers.</p><p><b>RESULTS</b>Morphologically, hAD-MSCs were induced to differentiate into neural stem/progenitor cells and expressed specific neural markers. After being co-cultured with embryonic chick otic vesicle cells, hAD-MSCs expressed specific surface markers of inner ear hair cells.</p><p><b>CONCLUSIONS</b>hAD-MSCs can be directionally induced to differentiate towards hair cell-like cells in vitro.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Diferenciação Celular
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Células Cultivadas
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Tecido Adiposo
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Técnicas de Cocultura
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Técnicas de Cultura de Células
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Biologia Celular
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Células-Tronco Mesenquimais
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Células Ciliadas Auditivas Internas
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Métodos
Limite:
Animals
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Humans
Idioma:
Zh
Revista:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
Ano de publicação:
2009
Tipo de documento:
Article