Effects of bicyclol on renal PAI-1 expression in rats with uniliteral ureteral obstruction / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 509-513, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-339609
ABSTRACT
<p><b>OBJECTIVE</b>To explore the protective effects of bicyclol against renal interstitial fibrosis and possible mechanisms of the protection.</p><p><b>METHODS</b>Eighty-one Sprague-Dawley (SD) rats were randomly assigned to a sham-operated group and UUO groups with and without bicyclol treatment. A rat model of renal interstitial fibrosis was prepared by unilateral ureteral obstruction (UUO). Renal tissues were examined by hematoxylin & eosin and Masson staining on 7, 14 and 21 days. Immunhistochemistry was used for determining plasminogen activator inhibitor-1(PAI-1) expression in the renal interstitium. PAI-1 mRNA expression in renal tissues was semi-quantitatively determined by reverse transcription-polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>The relative areas of renal interstitial fibrosis in the bicyclol-treated UUO group 7, 14 and 21 days after operation were (9.6 ± 0.6)%, (16.8 ± 0.8)% and (33.6 ± 1.6)% respectively, which were significantly lower than those in the untreated UUO group [13.0 ± 0.7)%, (25.8 ± 1.5)% and (53.2 ± 2.5)% respectively] (P<0.05). The levels of protein and mRNA expression of PAI-1 in the bicyclol-treated UUO group decreased significantly compared with those in the untreated UUO group 7, 14 and 21 days after operation (P<0.05).</p><p><b>CONCLUSIONS</b>Bicyclol can alleviate renal interstitial injury and renal interstitial fibrosis caused by UUO in rats, possibly through a downregulation of renal PAI-1 expression.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Obstrução Ureteral
/
Compostos de Bifenilo
/
Fibrose
/
RNA Mensageiro
/
Química
/
Ratos Sprague-Dawley
/
Inibidor 1 de Ativador de Plasminogênio
/
Usos Terapêuticos
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Contemporary Pediatrics
Ano de publicação:
2011
Tipo de documento:
Artigo
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