Establishment of the model of oxygen-glucose deprivation in vitro rat hippocampal neurons / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 197-200, 2003.
Article
em Zh
| WPRIM
| ID: wpr-339641
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>AIM</b>To establish the model of oxygen-glucose deprivation in vitro rat hippocampal neurons.</p><p><b>METHODS</b>The hippocampal neurons cultured for 12 d were exposed to combined oxygen-glucose deprivation for 0.5-4 h and then cultured with original medium in normoxia for 24 h. Auto-biochemical analyzer determined LDH activity. The change of neuronal morphology and neuron survival were observed by converted contrast microscope and assessed by photography analysis system. Neuron apoptosis was detected by using the terminal deoxynucleotidyl transferase-mediated biotinylated deoxyuridine triphosphate nickel end labeling (TUNEL) method.</p><p><b>RESULTS</b>The neurons swelled, LDH release increased and neuron survival decreased after gradually oxygen-glucose deprivation. The percentage of apoptosis increased obviously 24 h after recovering the supply of oxygen and glucose.</p><p><b>CONCLUSION</b>The model of oxygen-glucose deprivation in vitro rat hippocampal neurons is established successfully by using the modified ACSF (artificial cerebral spinal fluid) with serum and glucose free.</p>
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Índice:
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Assunto principal:
Oxigênio
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Fisiologia
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Hipóxia Celular
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Células Cultivadas
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Ratos Wistar
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Biologia Celular
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Glucose
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Hipocampo
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Animais Recém-Nascidos
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Neurônios
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2003
Tipo de documento:
Article