Effects of sodium hydrogen exchanger-1 hammerhead ribozyme on proliferation of pulmonary artery smooth muscle cells in rats in vitro / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 39-42, 2003.
Article
em Zh
| WPRIM
| ID: wpr-339685
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WPRO
ABSTRACT
<p><b>AIM</b>To explore the effects of sodium hydrogen exchanger (NHE-1) specific hammerhead ribozyme on the expression and activity of NHE-1 and pHi in pulmonary artery smooth muscle cells (PASMCs) in rats, and its role in PASMCs proliferation.</p><p><b>METHODS</b>According to the secondary structure of NHE-1 mRNA in rats, NHE-1 specific hammerhead ribozyme was designed with the assistance of computer. The recombinant vector of retroviral plasmid pLXSN and NHE-1 hammerhead ribozyme, PRZ, was transfected into the cultured PASMCs. G418 resistant cell clones were screened with 60 microg/ml G418. Then, the expression of NHE-1 mRNA was detected by RT-PCR, intracellular pH was measured with fluorescent probe-BCECF, 22Na and 3H-TdR incorporation were determined respectively.</p><p><b>RESULTS</b>Compared with the cells transfected with pLXSN and non-transfected cells, NHE-1 mRNA, pHi value, 3H-TdR and 22Na incorporation decreased significantly in cells transfected with recombinant vector PRZ. No significance was found between the pLXSN transfected group and non-transfected group.</p><p><b>CONCLUSION</b>NHE-1 hammerhead ribozyme can cleave the target RNA specifically, reduce the expression of NHE-1 mRNA, induce intracellular acidosis and consequently prohibit the proliferation of PASMCs.</p>
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Índice:
WPRIM
Assunto principal:
Patologia
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Artéria Pulmonar
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RNA Mensageiro
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Transfecção
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RNA Catalítico
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Trocadores de Sódio-Hidrogênio
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Biologia Celular
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Miócitos de Músculo Liso
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Proliferação de Células
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Trocador 1 de Sódio-Hidrogênio
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2003
Tipo de documento:
Article