Mechanisms of protection effect of bcl-2 gene transfection on heat-stressed cardiomyocytes / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 347-349, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-339718
ABSTRACT
<p><b>AIM</b>To study the mechanisms of protection of bcl-2 gene transfection against heat-stressed cardiomyocytes.</p><p><b>METHODS</b>Cardiomyocytes were isolated and cultured. bcl-2 was transfected into cardiomyocytes with Lipofectamine transfection methods. The cardiomyocytes were stressed by heat. The change of H+ -ATPase synthesis activity of cardiomyocytes mitochondria caused by bcl-2 transfection was measured by chemical radiation method. The changes of Caspase 3 activity of cardiomyocytes caused by bcl-2 transfection was measured by fluorometric analysis.</p><p><b>RESULTS</b>bcl-2 transfection could increase the H+ -ATPase synthesis activity of cardiomyocytes mitochondria under heat stress at 41 degrees C and 43 degrees C and could decrease the Caspase 3 activity of cardiomyocytes under heat stress at 41 degrees C and 43 degrees C.</p><p><b>CONCLUSION</b>The protection effect of bcl-2 transfection on heat-stressed cardiomyocytes may be associated with preserved H+ ATPase synthesis activity of cardiomyocytes mitochondria and the activity of Caspase 3 of cardiomyocytes.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Transfecção
/
Células Cultivadas
/
ATPases Translocadoras de Prótons
/
Resposta ao Choque Térmico
/
Genes bcl-2
/
Biologia Celular
/
Miócitos Cardíacos
/
Caspase 3
/
Genética
/
Metabolismo
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2002
Tipo de documento:
Artigo
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