Effect of PG0839 gene of Porphyromonas gingivalis on inflammatory cytokine in human oral epidermoid carcinoma KB cell / 中华口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of PG0839 gene form Porphyromonas gingivalis (Pg) on inflammatory cytokine expression in human oral epidermoid carcinoma KB cell.</p><p><b>METHODS</b>A mutant in the PG0839 gene of Pg was created by insertional inactivation. Group 1 was chanllenged with PgW83 strain, group 2 with PG0839-defective mutant, and the control group with Dulbecco's modified Eagle's medium only. KB cells were co-cultured with presence of bacteria for 24 h. At the time point of 0.5, 2, 6, 12 and 24 h, cells were stored in Trizol. The mRNA expression of interleukin-1β (IL-1β) and Toll like recepector-4 (TLR-4) was examined by reverse transcription polymerase chain reaction.</p><p><b>RESULTS</b>At 2 h and 6 h, IL-1β mRNA expression was lower in group 2 than in group 1 (2 h 0.31 ± 0.11 versus 0.95 ± 0.48, P < 0.05; 6 h 0.57 ± 0.20 versus 1.29 ± 0.55, P < 0.05). At 0.5 h and 6 h, TLR-4 mRNA expression was lower in group 2 than in group 1 (0.5 h 0.20 ± 0.09 versus 0.58 ± 0.09, P < 0.05; 6 h 0.34 ± 0.04 versus 0.71 ± 0.18, P < 0.05).</p><p><b>CONCLUSIONS</b>PG0839 gene may play an important role in Pg-induced inflammatory effects of KB cell.</p>