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The 2A protease of enterovirus 71 cleaves nup62 to inhibit nuclear transport / 病毒学报
Chinese Journal of Virology ; (6): 421-425, 2013.
Artigo em Chinês | WPRIM | ID: wpr-339934
ABSTRACT
To study the impact of the enterovirus 71(EV71) on the nuclear transport mechanism,The pGFP-NLS vector with nuclear location signal(NLS) was constructed, RD cells transfected by the pGFP-NLS vector were inoculated with the EV71 or cotransfected by EV71-2A vector. The results showed that GFP protein with NLS was expressed in the cytoplasm due to the inhibition of nuclear transport. In order to further study the mechanism of the EV71 to prevent nuclear transport,Nup62 was detected by Western blotting after RD cells were infected with EV71 or transfected by EV71-2A vector. The results showed that decreased expression of Nup62 could be detected after infection with EV71 and transfection by EV71-2A vector. This study demonstrates that the cleavage of Nup62 by EV71 2A protease may be the mechanism of nuclear transport inhibition.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Peptídeo Hidrolases / Virologia / Proteínas Recombinantes de Fusão / Glicoproteínas de Membrana / Transfecção / Regulação Viral da Expressão Gênica / Núcleo Celular / Sinais de Localização Nuclear / Transporte Ativo do Núcleo Celular / Enterovirus Humano A Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Virology Ano de publicação: 2013 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Peptídeo Hidrolases / Virologia / Proteínas Recombinantes de Fusão / Glicoproteínas de Membrana / Transfecção / Regulação Viral da Expressão Gênica / Núcleo Celular / Sinais de Localização Nuclear / Transporte Ativo do Núcleo Celular / Enterovirus Humano A Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Virology Ano de publicação: 2013 Tipo de documento: Artigo