Analysis of full-length gene sequence of rabies vaccine virus aG strain / 病毒学报
Chinese Journal of Virology
;
(6): 404-409, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-339937
ABSTRACT
To sequence and analyze the full-length gene sequence of rabies vaccine virus aG strain. The full-length gene sequence of aG strain was amplified by RT-PCR by 8 fragments,each PCR product was cloned into vector pGEM-T respectively, sequenced and assemblied; The 5' leader sequence was sequenced with method of 5' RACE. The homology between aG and other rabies vaccine virus was analyzed by using DNAstar and Mega4. 0 software. aG strain was 11 925nt(GenBank accession number JN234411) in length and belonged to the genotype I . The Bioinformatics revealed that the homology showed disparation form different rabies vaccine virus. the full-length gene sequence of rabies vaccine virus aG strain provided a support for perfecting the standard for quality control of virus strains for production of rabies vaccine for human use in China.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Raiva
/
Vírus da Raiva
/
Especificidade da Espécie
/
Virologia
/
Dados de Sequência Molecular
/
Vacina Antirrábica
/
Sequência de Bases
/
China
/
Sequência de Aminoácidos
Limite:
Humanos
País/Região como assunto:
Ásia
Idioma:
Chinês
Revista:
Chinese Journal of Virology
Ano de publicação:
2013
Tipo de documento:
Artigo
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