Inhibition of proliferation of H5N1 subtype AIV in CEF by chemosynthetic siRNA / 病毒学报
Chinese Journal of Virology
;
(6): 386-391, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-339940
ABSTRACT
In order to study the proliferation inhibition effect of H5N1 subtype avian influenza virus (AIV) with small interfere RNA (siRNA), a total of 4 siRNAs were designed in accordance with the NP and PA genes of H5N1 subtype AIV, the siRNAs were then transfected to chicken embryo fibroblast(CEF), CEF was infected with H5N1 subtype AIV after 6 hrs. Virus titer of cell supernatant was tested at 16-56hrs post infection, and pathological changes of the cells was observed; mRNA levels of NP, PA, HA and p13-actin gene were tested at 36hrs post infection. The results showed that these 4 siRNAs could inhibit the prolif-eration of H5N1 subtype AIV in CEF in varying degrees, and one siRNA targeting PA was best per-formed. The experimental results also showed that the inhibition effect was decreased with the time prolonged. This research provides a basis for further studying RNAi on AIV prevention and control.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Proteínas Virais
/
Virologia
/
Replicação Viral
/
RNA Polimerase Dependente de RNA
/
Transfecção
/
Proteínas do Core Viral
/
Actinas
/
Proteínas de Ligação a RNA
/
Organismos Livres de Patógenos Específicos
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Virology
Ano de publicação:
2013
Tipo de documento:
Artigo
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