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Proliferation characteristics of a PK-15 cell-adapted strain of porcine parvovirus / 病毒学报
Chinese Journal of Virology ; (6): 357-363, 2013.
Artigo em Chinês | WPRIM | ID: wpr-339945
ABSTRACT
To study the proliferation characteristics of PPV in differently infected way and the variance of concentrations in different cells. A strain of porcine parvovirus(PPV) was adapted to PK-15 cells, and a Real-time fluorescent quantitative PCR (FQ-PCR) assay was developed based on the specific region of the NS1 gene of PPV to quantify the PPV. The FQ-PCR was used to measure the viral concentration of virus-infected cells by simultaneous or step by step inoculation and plot one-step growth curves. The proliferation characteristics of PPV strain in different cells lines (HeLa, MDBK, PK-15 ,ST, F81, BHK-21 and Marc-145) was also compared. The results showed the PK-15 cell -adapted strain of PPV produced CPE after 12 passages, and maintained stable CPE at the following 10 messages. The one-step growth curve showed that the virus concentration of simultaneous inoculation was higher than that of the step-by-step inoculation, and the proliferation cycle of step-by-step inoculation was shorter. The proliferation ability of PPV strain in different cells showed that CPE appeared first inPK-15, followed by ST, HeLa and MDBK, and the virus concentration was highest in ST, followed byPK-15, MDBK and HeLa. NO proliferation was observed in F81, BHK-21 and Marc-145 cells. These findings lay a material foundation for the basic researches on PPV and the development of vaccine.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fisiologia / Suínos / Proteínas Virais / Virologia / Replicação Viral / DNA Viral / Linhagem Celular / Reprodutibilidade dos Testes / Sensibilidade e Especificidade / Haplorrinos Tipo de estudo: Estudo diagnóstico Limite: Animais / Feminino / Humanos / Masculino Idioma: Chinês Revista: Chinese Journal of Virology Ano de publicação: 2013 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fisiologia / Suínos / Proteínas Virais / Virologia / Replicação Viral / DNA Viral / Linhagem Celular / Reprodutibilidade dos Testes / Sensibilidade e Especificidade / Haplorrinos Tipo de estudo: Estudo diagnóstico Limite: Animais / Feminino / Humanos / Masculino Idioma: Chinês Revista: Chinese Journal of Virology Ano de publicação: 2013 Tipo de documento: Artigo