Genotype features of norovirus associated with acute gastroenteritis through sentinel surveillance in Guizhou Province, 2011 / 病毒学报

ABSTRACT

To study the genotype of Norovirus associated with acute gastroenteritis in Guizhou Province 2011, the patients' fecal specimens were collected from the Guizhou Province People's Hospital in the period of May to December 2011. Noroviruses in specimens were detected by a real-time reverse transcription polymerase chain reaction (Real-time RT-PCR). VP1 genes of norovirus-positive strains were then cloned and sequenced. Out of 70 clinical samples, the positive rates for norovirus G I (1 strain) and G II (34 strains) were 1.43% and 48.57, respectively. The VP1 sequencing results of seven norovirus G II showed thatthree strains were genotype G II . 4 and four strains were genotype G II . 3 Those genotype GIL . 4 strains were new variants (GII . 4 2011),closest to GII . 4 2006b variant. One amino acid appeared back mutation. Those genotype G II . 3 strains were divided into 2 gene clusters. One cluster was closest to Korean strain (HM635118) and Shanghai strain(GU991355). One cluster was closest to Japaness strain (AB629943) and 2007 Indian strain (EU921389), Four amino acids appeared back mutations.