Functional analysis of the late expression factor genes of plutella xylostella granulovirus / 病毒学报
Chinese Journal of Virology
;
(6): 560-566, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-340005
ABSTRACT
Plutella xylostella granulovirus (PlxyGV) contains homologs of 15 Autographa californica MNPV (AcMNPV) late expression factor (lef) genes. The prospective products of 14 PlxyGV lef genes (ie-0 is not included) share 13%-53% amino acid similarity with their corresponding homologs of AcMNPV, among which LEF-9, LEF-8 and P47, three subunits of the virus-encoded RNA polymerase, share 49%, 53% and 46% sequence identity, respectively. In this study, an established transient expression system was used to test the ability of the PlxyGV LEFs to activate an AcMNPV vp39 promoter-driven reporter gene in SF9 cells. It was shown that PlxyGV le f-2 replaced the corresponding AcMNPV gene and exhibited partial activity in the context of the remaining set of AcMNPV le fs. PlxyGV LEF-2 was found to contain additional 100aa and 70aa at the C-terminus in comparison with the LEF-2 of other GVs and lepidopteran NPVs respectively.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas Virais
/
Virologia
/
Dados de Sequência Molecular
/
Regulação Viral da Expressão Gênica
/
Química
/
Alinhamento de Sequência
/
Sequência de Aminoácidos
/
Nucleopoliedrovírus
/
Granulovirus
/
Genética
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Virology
Ano de publicação:
2012
Tipo de documento:
Artigo
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