Inhibition of virus infection in coxsackievirus-induced myocarditis model by RNA interference / 病毒学报
Chinese Journal of Virology
;
(6): 527-530, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-340011
ABSTRACT
To evaluate the effects of lentivirus-delivered short hairpin RNA (shRNA) on CVB3 infection in an animal model by RNA interference technique, we constructed a recombinant lentivirus expressing shRNA-3753 against the viral genome region 3753-3771, then transduced Lenti-sh3753 into mice infected with CVB3. We evaluated the antiviral ability of lenti-sh3753 by cytopathic effect (CPE), viral plaque assay and histological analysis of mice hearts. The results showed that Lenti-sh3753 exhibited a significant protective effect on cell viability and reduction of viral titers in supernatant of cell culture by specific inhibition on viral replication. Lenti-sh3753 also prolonged the mice survival and limited the viral production in mice hearts. These data proposed that Lenti-sh3753 can effectively inhibit CVB3 infection in a coxsackievirus-induced myocarditis model, suggesting its potential role in prevention and therapy of viral diseases.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Virologia
/
Replicação Viral
/
RNA Viral
/
Regulação para Baixo
/
Enterovirus Humano B
/
Infecções por Coxsackievirus
/
RNA Interferente Pequeno
/
Interferência de RNA
/
Usos Terapêuticos
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Virology
Ano de publicação:
2012
Tipo de documento:
Artigo
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