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Effects of extremely low frequency electromagnetic fields on the level of c-fos mRNA in brain and liver of mouse / 中华劳动卫生职业病杂志
Article em Zh | WPRIM | ID: wpr-340044
Biblioteca responsável: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of extremely low frequency electromagnetic fields (ELF EMFs) on c-fos gene expression in mouse brain and liver tissues.</p><p><b>METHODS</b>Mice were exposed to 50 Hz sinusoidal 0.2 mT or 6.0 mT electromagnetic field for 2 weeks or 4 weeks. Competitive RT-PCR method was used to measure c-fos mRNA level.</p><p><b>RESULTS</b>After exposure to 0.2 mT or 6.0 mT field for 2 weeks, c-fos mRNA levels in brain tissue [(0.0178 +/- 0.0076) amol/120 ng cDNA and (0.0092 +/- 0.0042) amol/120 ng cDNA respectively] were higher than that of control level [(0.0012 +/- 0.0005) amol/120 ng cDNA] (P < 0.05). In liver tissue c-fos mRNA levels [(0.0117 +/- 0.0055) amol/120 ng cDNA and (0.0148 +/- 0.0162) amol/120 ng cDNA respectively] were also higher than that of control level [(0.0005 +/- 0.0005) amol/120 ng cDNA] (P < 0.05). After exposure to 0.2 mT or 6.0 mT field for 4 weeks, c-fos mRNA levels in brain tissue [(0.0100 +/- 0.0054) amol/120 ng cDNA and (0.0198 +/- 0.0079) amol/120 ng cDNA respectively] were higher than that of control level [(0.0015 +/- 0.0008) amol/120 ng cDNA] (P < 0.05). In liver tissue the exposure induced much higher expression level [(0.0173 +/- 0.0122) amol/120 ng cDNA and (0.0133 +/- 0.0090) amol/120 ng cDNA respectively] while no expression was found in the control.</p><p><b>CONCLUSION</b>Exposure to 50 Hz electromagnetic fields may induce up-regulation of c-fos transcription in mouse brain and liver tissue.</p>
Assuntos
Texto completo: 1 Índice: WPRIM Assunto principal: Efeitos da Radiação / Fatores de Tempo / Encéfalo / RNA Mensageiro / Regulação da Expressão Gênica / Genes fos / Reação em Cadeia da Polimerase Via Transcriptase Reversa / Relação Dose-Resposta à Radiação / Campos Eletromagnéticos / Genética Limite: Animals Idioma: Zh Revista: Chinese Journal of Industrial Hygiene and Occupational Diseases Ano de publicação: 2003 Tipo de documento: Article
Texto completo: 1 Índice: WPRIM Assunto principal: Efeitos da Radiação / Fatores de Tempo / Encéfalo / RNA Mensageiro / Regulação da Expressão Gênica / Genes fos / Reação em Cadeia da Polimerase Via Transcriptase Reversa / Relação Dose-Resposta à Radiação / Campos Eletromagnéticos / Genética Limite: Animals Idioma: Zh Revista: Chinese Journal of Industrial Hygiene and Occupational Diseases Ano de publicação: 2003 Tipo de documento: Article