Study on the difference in DNA damage of human lymphocyte caused by arsenicals / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 327-330, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-340074
ABSTRACT
<p><b>OBJECTIVE</b>To explore whether there is difference in arsenicals-induced DNA damage of human lymphocyte.</p><p><b>METHODS</b>Lymphocyte were sterilely collected from healthy donor and exposed to sodium arsenite (AsIII), sodium arsenate(AsV) and methyl sodium arsenate(MAsv) at 1,5,10,20 and 50 mumol/L. After incubation of 24 hours, cells were collected by centrifugation and DNA damage was detected by single cell gel electrophoresis (SCGE).</p><p><b>RESULTS</b>The comet frequency distribution of all groups except 1 mumol/L group of MAsV were significantly different from that of control. The comet length of all groups except 1 mumol/L group of AsV and 1.5 mumol/L groups of MAsV were significantly higher than that of control. There were correlations between the doses of arsenicals and the ratios of comet cell or length of comet(rAsIII = 0.8134, rAsV = 0.8734, rMAsV = 0.8994).</p><p><b>CONCLUSION</b>DNA damage in human lymphocyte were induced by all the three arsenicals. A dose-effect relationship was observed between exposure doses of the same arsenical and DNA damage. With different arsenicals but the same exposure dose, the DNA damage level was as follow AsIII > AsV > MAsV.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Arseniatos
/
Dano ao DNA
/
Linfócitos
/
Arsenitos
/
Ensaio Cometa
/
Relação Dose-Resposta a Droga
/
Toxicidade
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Ano de publicação:
2002
Tipo de documento:
Artigo
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