Expression and clinical significance of VEGF and apoptosis in frozen-thawed mouse ovaries after transplantation / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 172-176, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-340203
ABSTRACT
<p><b>OBJECTIVE</b>To investigate expression and clinical significance of VEGF and apoptosis in frozen-thawed mouse ovaries after transplantation.</p><p><b>METHODS</b>Ovaries from B6C2F1 (C57BL/6j x BALB/c) 4 week old mice were cryopreservation and the thawed ovaries were xenografted into kidney capsules of 8-12 week old adult male mice. The grafted were recovered 1 d, 2 d and 7 d after transplantation respectively, the grafts and frozen-thawed were removed for follicle counting and immunohistochemically, ultrastructure, and detection of the mRNA expression of vascular endothelial growth factor(VEGF).</p><p><b>RESULTS</b>The follicle numbers were decreased gradually after transplantation,the cell apoptosis increased especially in 48 h after transplantation. Transmission electron microscopy (TEM) showed the tissue damaged was severest 48 h after transplantation; the expression of VEGF increased after transplantation, peaked on day 7, the mRNA expression of VEGF120 and VEGF188 was more on 48 h after transplantation, decreased on day 7 (P < 0.05).</p><p><b>CONCLUSION</b>The number of follicles was decreased after transplantation, the apoptosis index was increased especially in 48 h after transplantation; the expression of VEGF increased after transplantation, an increase in the VEGF188 and VEGF164 isoform might suggest the positive effect in the early stages of angiogenesis.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Preservação de Órgãos
/
Ovário
/
Patologia
/
Fisiologia
/
Transplante
/
Criopreservação
/
Apoptose
/
Fator A de Crescimento do Endotélio Vascular
/
Folículo Ovariano
/
Metabolismo
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2010
Tipo de documento:
Artigo
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