Mechanism of peripheral blood mononuclear cell invasion by HBV on artificial immunization in newborns / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1380-1382, 2002.
Artigo
em Inglês
| WPRIM
| ID: wpr-340325
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect and mechanism of the peripheral blood mononuclear cell (PBMC) invasion by HBV on artificial immunization in newborns.</p><p><b>METHODS</b>Fifty-two newborns of HBsAg positive mothers were immunized with HBIG (hepatitis B immunoglobulin) and HBVac (hepatitis B vaccine) and were followed up for 7 months. The newborns' HBV-DNA in serum and in the PBMCs was detected with nested-PCR; anti-HBs was tested with solid phase radioimmunoassay (SP-RIA). PBMCs isolated from newborn peripheral blood were incubated in the presence of PHA or purified HBsAg. Interleukin-2 (IL-2) level in culture supernatants of activated cells was detected by ELISA.</p><p><b>RESULTS</b>The failure rate of immunization was higher in infants with positive HBV-DNA in PBMCs than those with negative HBV-DNA (P < 0.05); IL-2 level in PBMC culture supernatants was lower in former than in the latter and in normal controls (P < 0.05). The level of IL-2 in the immunization failure newborns was lower than that in the successfully immunized newborns and in normal controls (P < 0.05).</p><p><b>CONCLUSIONS</b>Intrauterine invasion of PBMCs by HBV is one of the important reasons for immunization failure in newborns. IL-2 production is closely related to the invasion of PBMCs by HBV, which may contribute to the failure of artificial immunization in newborns.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Virologia
/
Sangue
/
DNA Viral
/
Imunoglobulinas
/
Leucócitos Mononucleares
/
Vírus da Hepatite B
/
Imunização
/
Interleucina-2
/
Vacinas contra Hepatite B
Limite:
Humanos
/
Recém-Nascido
Idioma:
Inglês
Revista:
Chinese Medical Journal
Ano de publicação:
2002
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS