The intraspecific difference of the triose phosphate isomerase (tim) gene from Giardia lamblia / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 763-766, 2002.
Artigo
em Inglês
| WPRIM
| ID: wpr-340419
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the intraspecific difference of the triose phosphate isomerase (tim) gene from Giardia lamblia (G. lamblia).</p><p><b>METHODS</b>Total genomic DNA of G. lamblia was extracted and partial fragments of the triose phosphate isomerase (tim) gene were amplified by polymerase chain reaction (PCR). All nucleotide sequences were analyzed by using a phylogenetic analysis, which was constructed with parsimony and Neighbor-joining (N-J) methods.</p><p><b>RESULTS</b>A total of 124 variable sites (23% of all sequences detected) was defined, most of which were found at the silent sites of codons. Two similar phylogenetic trees were constructed, subdividing 16 Giardia isolates into two groups.</p><p><b>CONCLUSION</b>The genetic diversity of G. lamblia appeared to be little affected by factors of both host and geography, while natural-selection played an important role in DNA molecular evolution level of the tim gene. The tim gene may be considered a very useful genetic marker of the population genetic structure of G. lamblia.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Especificidade da Espécie
/
Triose-Fosfato Isomerase
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Homologia de Sequência do Ácido Nucleico
/
Química
/
Reação em Cadeia da Polimerase
/
DNA de Protozoário
/
Alinhamento de Sequência
Limite:
Animais
Idioma:
Inglês
Revista:
Chinese Medical Journal
Ano de publicação:
2002
Tipo de documento:
Artigo
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