Endothelin-1, an important mitogen of smooth muscle cells of spontaneously hypertensive rats / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 750-752, 2002.
Artigo
em Inglês
| WPRIM
| ID: wpr-340422
ABSTRACT
<p><b>OBJECTIVE</b>To study the features of vascular smooth muscle cell (VSMC) proliferation induced by endothelin-1 (ET-1).</p><p><b>METHODS</b>VSMCs of spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats were cultured and treated with ET-1. Basic fibroblast growth factor (bFGF) gene expression was measured using both Northern blot and an enzyme-linked immunoassay.</p><p><b>RESULTS</b>ET-1 resulted in an increase in bFGF transcripts at 8 - 24 h; bFGF levels were significantly higher in VSMCs treated with ET-1 than in those not treated. However, VSMCs growth responses in SHR and WKY were different. Smooth muscle cells of SHR were hyper-responsive to ET-1. Maximal bFGF mRNA levels were elevated 3.5-fold at 4 h of stimulation in WKY and 8-fold at 8h in SHR4. Moreover, the proliferation of VSMCs induced by ET-1 was inhibited by antisense phosphorothioate oligodeoxynucleotides (10 micromol/L AS-bFGF) but not sense bFGF oligomers at the same concentrations, being reduced by 80% in SHR and 40% in WKY vs control, respectively. Furthermore, the effect of AS-bFGF oligomers on SHR SMC proliferation is significantly greater than on WKY SMC proliferation.</p><p><b>CONCLUSION</b>ET-1 may be required for exaggerated vascular growth responses in SHR and bFGF may be involved.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Oligonucleotídeos
/
Farmacologia
/
Ratos Endogâmicos SHR
/
Ratos Endogâmicos WKY
/
Fatores de Tempo
/
RNA Mensageiro
/
Divisão Celular
/
Células Cultivadas
/
Regulação da Expressão Gênica
/
DNA Antissenso
Limite:
Animais
Idioma:
Inglês
Revista:
Chinese Medical Journal
Ano de publicação:
2002
Tipo de documento:
Artigo
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