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Effect of rosiglitazone on tumor necrosis factor-alpha-induced nuclear factor-kappaB and coupling factor 6 expressions in human umbilical vein endothelial cells / 南方医科大学学报
Journal of Southern Medical University ; (12): 1642-1645, 2008.
Artigo em Chinês | WPRIM | ID: wpr-340758
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of rosiglitazone on the expression of nuclear factor-kappaB (NF-kappaB) and coupling factor 6 (CF6) induced by tumor necrosis factor-alpha (TNF-alpha) in cultured human umbilical vein endothelial cells (HUVEC).</p><p><b>METHODS</b>Cultured HUVEC of passage 3-5 were stimulated with TNF-alpha and then cultured in the presence of rosiglitazone. The expression of CF6 and NF-kappaB subunit p65 were evaluated by immunocytochemistical method.</p><p><b>RESULTS</b>Pretreatment of HUVECs with rosiglitazone inhibited TNF-alpha-induced expression of CF6 in a dose-dependent manner. The activation of CF6 stimulated by TNF-alpha was suppressed by ROS in a dose-dependent manner.</p><p><b>CONCLUSION</b>TNF-alpha-induced enhancement of the gene expression and release of CF6 is mediated by activation of NF-kappaB signaling pathway. ROS can inhibit the activation of IKK, block NF-kappaB signaling pathway and inhibit the expression of CF6, which may be the mechanism underlying the action of TZDs on hypertension.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fatores Acopladores da Fosforilação Oxidativa / Farmacologia / Veias Umbilicais / Imuno-Histoquímica / Células Cultivadas / NF-kappa B / Fator de Necrose Tumoral alfa / Biologia Celular / ATPases Mitocondriais Próton-Translocadoras / Células Endoteliais Limite: Humanos Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2008 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fatores Acopladores da Fosforilação Oxidativa / Farmacologia / Veias Umbilicais / Imuno-Histoquímica / Células Cultivadas / NF-kappa B / Fator de Necrose Tumoral alfa / Biologia Celular / ATPases Mitocondriais Próton-Translocadoras / Células Endoteliais Limite: Humanos Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2008 Tipo de documento: Artigo