Construction of a recombinant eukaryotic vector of human intestinal trefoil factor and its expression in 293-T cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1630-1633, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-340762
ABSTRACT
<p><b>OBJECTIVE</b>To clone human intestinal trefoil factor (hITF/hTFF3) gene into an eukaryotic expression vector for its expression in eukaryotic cells.</p><p><b>METHODS</b>The total RNA was extracted from normal human colon mucosa, and transcribed into cDNAs using RT-PCR. hTFF3 gene was amplified by PCR and ligated into pGEMT vector by TA cloning method. After sequencing, the hTFF3 gene was transfered into the eukaryotic expression vector pCMV5-myc. The recombinant vector was transfected into 293-T cells, and the expression of the recombinant protein was detected by Western blotting.</p><p><b>RESULTS AND CONCLUSION</b>hTFF3 gene was successfully cloned from normal human colon mucosa. The vector pCMV5-myc-hTFF3 was reconstructed, and in 293-T cells transfected with the vector, hTFF3 expression was detected by Western blotting.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Peptídeos
/
Proteínas Recombinantes
/
Transfecção
/
Linhagem Celular
/
Western Blotting
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Células Eucarióticas
/
Fator Trefoil-2
/
Fator Trefoil-3
/
Vetores Genéticos
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2008
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS