Preliminary study of the association between human thrombospondin and gastric cancer / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1546-1549, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-340782
ABSTRACT
<p><b>OBJECTIVE</b>To study the possible role of human thrombospondin (hPWTSR) in gastric cancer and explore its potential to serve as the target for gastric cancer diagnosis and intervention.</p><p><b>METHODS</b>Using pLexA-hPWTSR as the bait, a premade pB42AD-based fetal brain cDNA library was constructed to identify the interacting proteins. The expression pattern of hPWTSR in gastric cancer tissues and a gastric cancer cell line was observed to investigate the correlation between hPWTSR expression and the biological behaviors of the tumor. The possibility of hPWTSR as a potential gastric cancer marker was evaluated.</p><p><b>RESULTS</b>Fifty-seven independent clones were isolated from 107 clones screened. Sequence analysis indicated that the 57 positive clones represented the products of 12 genes. A RT-PCR-based expression pattern revealed that the expression of hPWTSR in gastric cancer tissues and a gastric cancer cell line was lower than that in the corresponding normal tissues, but no mutations were identified by the subsequent sequence analysis.</p><p><b>CONCLUSIONS</b>hPWTSR interacts with adhesion-related proteins and tumor-related genes, and its expression is lowered in gastric cancer tissues and gastric cancer cell line. hPWTSR might play a role in gastric cancer development, especially in metastasis and might be used as a potential gastric cancer marker. The exact functions of hPWTSR and its potential clinical value still await further study.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Ligação Proteica
/
Neoplasias Gástricas
/
Biomarcadores Tumorais
/
Regulação Neoplásica da Expressão Gênica
/
Trombospondinas
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Perfilação da Expressão Gênica
/
Linhagem Celular Tumoral
/
Genética
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2008
Tipo de documento:
Artigo
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