The effects of tensile strain and loading time on the secretion of IL-1 beta of human PDLF / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 652-656, 2002.
Article
em Zh
| WPRIM
| ID: wpr-340944
Biblioteca responsável:
WPRO
ABSTRACT
This study aims at exploring the effects of tensile strain and loading time on the secretion of IL-1 beta of human periodontal ligament fibroblast. Five tensile strain values including 0%, 8%, 12%, 16%, 20% and three loading time including 24 h, 48 h, 72 h are set in this study. The prepared cell samples are mounted on the self-devised loading apparatus in vitro. The content of IL-1 beta in each sample was determined using double-antibody ELISA. The secretion amount of IL-1 beta per day is directly proportional to the loading time and tensile strain value in tensile strain group of 8%, 12%, 16%. The secretion amount of IL-1 beta reaches its maximum at tensile strain value of 16% in loading time groups. Loaded strain for 24 h and 48 h, the secretion amount of IL-1 beta at tensile strain value of 20% is obviously more than that at value of 0%, but the amount already begin to decrease apparently. Loaded strain for 72 h, the secretion amount of IL-1 beta decreases to a great extent that it is less than the amount at strain value of 0%. The tensile strain stimulates the secretion of IL-1 beta by human periodontal ligament fibroblast when the strain is under normal physiological extent, but the stimulation effect fades out as time goes on.
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Índice:
WPRIM
Assunto principal:
Ligamento Periodontal
/
Resistência à Tração
/
Fatores de Tempo
/
Células Cultivadas
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Interleucina-1
/
Secreções Corporais
/
Biologia Celular
/
Fibroblastos
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2002
Tipo de documento:
Article