Upregulation of Phagocytic Clearance of Apoptotic Cells by Autoimmune Regulator / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
;
(6): 145-148, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-341107
ABSTRACT
To investigate the effect of autoimmune regulator(AIRE)on phagocytic clearance of apoptotic cells,a recombinant expression vector containing full-length human AIRE cDNA was transfected into 16HBE cells.After incubation with transfected 16HBE cells,engulfment of apoptotic HL-60 cells induced by camptothecin was detected by myeloperoxidase(MPO)staining.The change in the expression of Rac 1 in transfected 16HBE cells was determined by RT-PCR and Western blotting.The results showed that the phagocytosis percentage of the experimental group,the mock trans-fection group and the negative control group(non-apoptotic cells)was(25.50±3.67)%,(6.25±1.58)%and(1.0±0.67)%,respectively.Moreover,the expressions of Rac 1 mRNA and protein were up-regulated in AIRE-transfected 16HBE cells,suggesting that A1RE may function as a regulator in the phagocytic clearance of apoptotic cells by promoting the expression of Rac 1.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Ano de publicação:
2010
Tipo de documento:
Artigo
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