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Platelet-derived growth factor-BB inhibited p21(WAF1) expression partially through transforming growth factor-beta signalling system in vascular smooth muscle cell / 中华心血管病杂志
Chinese Journal of Cardiology ; (12): 160-165, 2010.
Artigo em Chinês | WPRIM | ID: wpr-341262
ABSTRACT
<p><b>OBJECTIVE</b>To assess if the modulating effect of platelet-derived growth factor (PDGF)-BB on p21(WAF1) was mediated by upregulating transforming growth factor (TGF)-beta(1) expression in vascular smooth muscle cells (VSMC).</p><p><b>METHODS</b>TGF-beta(1) mRNA and protein expressions were measured by reverse transcription-PCR and ELISA, the protein expressions of p21(WAF1) and the downstream TGF-beta signalling including TGF-beta type I receptor (ALK-5 in VSMC), Smurf2, pSmad2/3, Smad4, Smad7 were detected by Western blot.</p><p><b>RESULTS</b>PDGF-BB significantly upregulated the expressions of TGF-beta(1) at mRNA (0.79-fold) and protein (1.98-fold) levels in VSMC, significantly inhibited the expression of p21(WAF1) (-67 +/- 12)%, and enhanced the expressions of ALK-5, pSmad2/3, Smad4, Smurf2 protein by 1.21-fold, 0.95-fold, 0.69-fold and 2.55-fold respectively, inhibited Smad7 expression (-65 +/- 9)%, these alterations were partially restored by anti-TGF-beta(1) neutralizing antibody.</p><p><b>CONCLUSIONS</b>These findings suggested that PDGF-BB inhibited p21(WAF1) expression in VSMC partially through upregulating TGF-beta(1) expression via PDGF-BB and TGF-beta signalling pathways.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Fator de Crescimento Derivado de Plaquetas / Transdução de Sinais / Divisão Celular / Células / Ratos Sprague-Dawley / Proteínas Proto-Oncogênicas c-sis / Biologia Celular / Miócitos de Músculo Liso / Inibidor de Quinase Dependente de Ciclina p21 Limite: Animais Idioma: Chinês Revista: Chinese Journal of Cardiology Ano de publicação: 2010 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Fator de Crescimento Derivado de Plaquetas / Transdução de Sinais / Divisão Celular / Células / Ratos Sprague-Dawley / Proteínas Proto-Oncogênicas c-sis / Biologia Celular / Miócitos de Músculo Liso / Inibidor de Quinase Dependente de Ciclina p21 Limite: Animais Idioma: Chinês Revista: Chinese Journal of Cardiology Ano de publicação: 2010 Tipo de documento: Artigo