The relationship between histone acetylation modification and IFN-gamma responsive gene regulation / 中华肝脏病杂志

ABSTRACT

<p><b>OBJECTIVES</b>To study the role of histone modification in the regulation of IFN-gamma-activated gene using chromatin immunoprecipitation technique.</p><p><b>METHODS</b>Real-time PCR was used to measure the mRNA level of interferon-gamma-inducible protein 10 (IP-10) in Hela cells. Chromatin immunoprecipitation combined with Real-time PCR was used to check the histone H4 acetylation level at IFN-stimulated response element (ISRE) locus of IP-10 gene.</p><p><b>RESULTS</b>IP-10 was strongly activated by IFN-gamma. The histone H4 deacetylation happened at the ISRE locus when IP-10 was induced by IFN-gamma. The activation of IP-10 and the deacetylation of histone H4 at the ISRE site induced by IFN-gamma were inhibited or blocked by histone deacetylase (HDAC) inhibitor trichostatin A (TSA).</p><p><b>CONCLUSION</b>The histone H4 deacetylation at the ISRE site is related with the activation of IP-10 by IFN-gamma.</p>