Screening the hALR-interacting protein from the cDNA library of hepatocarcinoma cells and studying its biological functions / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 346-349, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-341368
ABSTRACT
<p><b>OBJECTIVES</b>To screen the hALR-interacting protein by phage-displayed technique and identify its biological activities.</p><p><b>METHODS</b>The specific phage clones that interacted with target protein hALR from a cDNA library of hepatocarcinoma cells were selected using the T7 phage-displayed technique. The acquired cDNA inserts were sequenced and analyzed by bioinformatic tools. The biological activities of the phage-displayed peptide affecting QGY hepatocarcinoma cells were studied using 3H-TdR method.</p><p><b>RESULTS</b>The cDNA inserts with 212 bp were acquired after 4 rounds of biopanning. They showed 100% homology with citron kinase. The phage-displayed peptide and the peptide combined with hALR affected QGY cells proliferation.</p><p><b>CONCLUSIONS</b>hALR-interacting peptide can be specifically screened by phage-displayed technique. Citron kinase that interacted with hALR potentially plays an important role in the proliferation of hepatocarcinoma cells.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Peptídeos
/
Ligação Proteica
/
Células Tumorais Cultivadas
/
Proteínas
/
Biblioteca Gênica
/
Carcinoma Hepatocelular
/
Biblioteca de Peptídeos
/
Genética
/
Neoplasias Hepáticas
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Hepatology
Ano de publicação:
2006
Tipo de documento:
Artigo
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