Screening for the serum differential proteins of renal cell carcinoma using magnetic beads-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 287-291, 2011.
Artigo
em Inglês
| WPRIM
| ID: wpr-341413
ABSTRACT
<p><b>OBJECTIVE</b>To screen for the differential protein peaks of renal cell carcinoma (RCC) using magnetic beads-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS).</p><p><b>METHODS</b>Serum proteins were profiled by magnetic beads (WCX) from 62 RCC patients and 37 patients with benign renal space-occupying lesions. Protein peaks were identified by MALDI-TOF-MS. Data were analyzed with Biomarker Wizard 3.1 and Biomarker Patterns Software 5.0. Diagnostic model for RCC was constructed based on 47 RCC cases and 26 patients with benign renal space-occupying lesions. The remaining 26 cases were evaluated with blind method.</p><p><b>RESULTS</b>Seven differential protein peaks related to RCC were identified (Pβ0.05). The diagnostic model for RCC constructed by the differential protein peaks (m/z 2945.35, 15340.8, 6984.51, and 5819.23) generated excellent separation between the RCC and control groups, with a sensitivity of 83.0% and the specificity of 84.6%. As validated by blind method, the model had a sensitivity of 80.0% and a specificity of 81.8%.</p><p><b>CONCLUSION</b>Differential protein peaks for RCC can be identified in serum by magnetic beads-based MALDI-TOF-MS, which is also valuable for the establishment of a RCC diagnostic model with a high sensitivity and specificity.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Sangue
/
Proteínas Sanguíneas
/
Carcinoma de Células Renais
/
Biomarcadores Tumorais
/
Sensibilidade e Especificidade
/
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
/
Proteômica
/
Diagnóstico
/
Métodos
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Limite:
Adulto
/
Idoso
/
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Acta Academiae Medicinae Sinicae
Ano de publicação:
2011
Tipo de documento:
Artigo
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