A rudimentary study of the acid fibroblast growth factor's plant expression vector construction and transformation tobacco / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 126-131, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-341668
ABSTRACT
Acid fibroblast growth factor (aFGF) has great potential in clinical application, but it is very expensive. In order to reduce the cost of production and to make full use of the merits integrated with plant bioreator, we have explored the aFGF in transgenic Tobacco expression. AFGF gene was inserted into plant expression vector pBI121; the acquired plants contained aFGF gene expression vector pBI121-TOAB-aF. Using Agrobacterium-mediated gene transformation of Tobacco and using transgenic Tobacco containing kanamycin and cephalosporin culture medium, we obtained kanamycin resistant transgenic Tobacco plants. PCR detection, RT-PCR detection and Western blot detection confirmed that foreign genes were successfully expressed in Tobacco. These data could serve as a theoretical foundation on which to use the plant bioreactor for production of aFGF.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Nicotiana
/
Proteínas Recombinantes
/
Fator 1 de Crescimento de Fibroblastos
/
Plantas Geneticamente Modificadas
/
Agrobacterium
/
Vetores Genéticos
/
Genética
/
Metabolismo
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2010
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS