Reversing effect of histamine on neuron death induced by N-methyl-D-aspartate / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 189-192, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-341910
ABSTRACT
<p><b>OBJECTIVE</b>To determine the effect of histamine on N-methyl-D-aspartate (NMDA) induced neuron death and to elucidate its mechanism.</p><p><b>METHODS</b>The primary cortical cell culture was adopted. Neuron morphology and MTT assay were used to evaluate the drugs effects.</p><p><b>RESULT</b>Histamine at doses of 10(-4) 10(-6) 10(-7) 10(-8) mol/L reversed the neuron death induced by NMDA (50 micromol/L) for 3 h. The protection of histamine peaked at doses of 10(-4) mol/L and 10(-7)mol/L. The effect of histamine of 10(-7) mol/L was reversed only by cimetidine an H(2)receptor antagonist. However, the effect of histamine of 10(-4) mol/L was reversed only by pyrilamine but not cimetidine.</p><p><b>CONCLUSION</b>Histamine could reduce neuron death induced by NMDA; its protection at a low dose might be mediated by H(2)receptor, and at a high dose by H(1)receptor.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fisiologia
/
Receptores Histamínicos H1
/
Receptores Histamínicos H2
/
Histamina
/
Células Cultivadas
/
N-Metilaspartato
/
Morte Celular
/
Ratos Sprague-Dawley
/
Fármacos Neuroprotetores
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2004
Tipo de documento:
Artigo
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