HPLC determination of zolmitriptan and its related substances / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 37-40, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-341946
ABSTRACT
<p><b>OBJECTIVE</b>To develop an analytical method and quality control for determination of zolmitriptan and related substances.</p><p><b>METHODS</b>Zolmitriptan and related substances were separated and determined on a shimadzu CLC-C(8) column (150 mm x 6 mm, 10 microm) with a mobile phase of acetonitrile-10 mmol/L phosphate buffer (2575 pH 7.5) and a flow-rate of 1 ml/min; the UV-VIS detector was operated at 229 nm.</p><p><b>RESULT</b>The limit of detection for the related substances was 0.5 ng on the zolmitriptan basis (S/N >3). Linear calibration curve was gene rated from 4 - 40 microg/ml with a correlation coefficient of 0.9999. The recovery rate of zolmitriptan was 99.1% with a standard deviation of 0.2%. The results of HPLC method were consistent with those of nonaqueous titration method.</p><p><b>CONCLUSION</b>HPLC method is a rapid sensitive and accurate method for the determination of zolmitriptan and its related substances.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Triptaminas
/
Cromatografia Líquida de Alta Pressão
/
Oxazolidinonas
Idioma:
Chinês
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2004
Tipo de documento:
Artigo
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