Correlation between basic expression level of NMDA receptor NR1 subunit protein in hippocampus and learning ability in rats / 浙江大学学报·医学版

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the relationship between basic expression level of NMDA receptor NR1 subunit protein in hippocampus and learning ability of rats.</p><p><b>METHODS</b>Using a novel-object recognition model and Morris water maze,the novel-object recognition ability and spatial memory of SD rats were ranked, and grouped as the high (top 20 %) and the low (bottom 20%), respectively. NR1 subunit protein levels in hippocampus were measured by quantitative immunoblotting with NR1 subunit specific antibody.</p><p><b>RESULT</b>The level of NR1 subunit protein in hippocampus in the high novel-object recognition ability group was 60% (P<0.01), higher than that in the low one, and in the high spatial memory group it was 45.4 % (P<0.05), higher than that in the low one, respectively.</p><p><b>CONCLUSION</b>The basic expression level of NR1 subunit protein in hippocampus is related to novel-object recognition ability and spatial memory of rats.</p>