Mutation of lambdapL/pR-cI857 system for production of bacterial ghost in Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1423-1430, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-342384
ABSTRACT
Bacterial ghost is intact envelope of Gram-negative bacteria, which is produced by the function of the lysis gene E from bacteriophage PhiX174. The expression of the lysis gene E is usually controlled by the thermosensitive lambdapL/pR-cI857 promoter. In this study, we described a mutation (T --> C) at the ninth nucleotide of the OR2 in the lambdapR promoter of the lambdapL/pR-cI857 system by overlap PCR. The bacteriolytic assay showed that the mutation in the lambdapL/pR-cI857 system enhanced the temperature of repressing the expression of gene E up to 37 degrees C. The lysis efficiency of altered lambdapR promoter in Escherichia coli DH5a and avian pathogenic E. coli DE17 was up to 99.9%. The expanded range of temperature will benefit for the production of bacterial ghost.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Bacteriólise
/
Proteínas Virais
/
Virologia
/
DNA Bacteriano
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Membrana Celular
/
Regulação da Expressão Gênica
/
Regiões Promotoras Genéticas
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2012
Tipo de documento:
Artigo
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