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Biocompatibility of surface modified PHBHHx with rat embryonic neural stem cells / 生物工程学报
Chinese Journal of Biotechnology ; (12): 1216-1226, 2012.
Artigo em Chinês | WPRIM | ID: wpr-342403
ABSTRACT
To study the attachment, proliferation and differentiation of neural stem cells (NSCs) on surface modified PHBHHx films and to establish the theory of PHBHHx application in NSCs-based brain tissue engineering. PHBHHx film was fabricated by a solution-casting method, and the morphology of the film was observed under scanning electron microscopy(SEM). The films were treated by NaOH or lipase, then the surface hydrophilic property was characterized using water contact angle measurement. NSCs were isolated from the cerebral cortex of rat embryos on embryonic day 14.5, and cultured on surface treated PHBHHx films. The morphology of NSCs attached on the film was visualized under SEM, and the survival and differentiation of NSCs were observed through immunocytochemical staining. Compared with the untreated PHBHHx films, the water contact angle of NaOH or lipase treated PHBHHx films decreased dramatically, and the number of NSCs attached significantly increased. NSCs survived well on treated PHBHHx films and differentiated into neurons and glial cells. The amelioration of hydrophilic property of PHBHHx film improved its biocompatibility with NSCs. PHBHHx can serve as a novel CNS tissue engineering biomaterial applied for NSCs transplantation, brain repairing and regeneration.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fisiologia / Propriedades de Superfície / Caproatos / Adesão Celular / Diferenciação Celular / Células Cultivadas / Córtex Cerebral / Química / Ácido 3-Hidroxibutírico / Materiais Revestidos Biocompatíveis Limite: Animais Idioma: Chinês Revista: Chinese Journal of Biotechnology Ano de publicação: 2012 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fisiologia / Propriedades de Superfície / Caproatos / Adesão Celular / Diferenciação Celular / Células Cultivadas / Córtex Cerebral / Química / Ácido 3-Hidroxibutírico / Materiais Revestidos Biocompatíveis Limite: Animais Idioma: Chinês Revista: Chinese Journal of Biotechnology Ano de publicação: 2012 Tipo de documento: Artigo