Quantification of the curing effects of phenanthridine on yeast prion PSI+ / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 737-746, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-342445
ABSTRACT
In order to quantify the curing effects of phenanthridine on yeast prion, we introduced semi-denaturing agarose gel electrophoresis and fluorescence recovery after photobleaching techniques to quantify the curing effects of phenanthridine on yeast prion at the protein and cellular levels with the [PSI+] yeast strain expressing GFP-Sup35p (NGMC). The results showed that these two approaches could precisely quantify the curing effects of phenanthridine on [PSI+] cells. After a treatment for 1 through 5 days with phenanthridine, the curing rates of [PSI+] cells were 0%, 0%, 51.7%, 87.5% and 94.4%, respectively. Meanwhile, we quantified the sizes of Sup35p polymers in phenanthridine induced pink phenotype cells. The aggregation status in 1-2 days phenanthridine treated cells were similar to those in [PSI+] cells, while the aggregation status in 3-5 days phenanthridine treated cells were similar to those in [psi(-)] cells.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fenantridinas
/
Saccharomyces cerevisiae
/
Simulação por Computador
/
Príons
/
Fatores de Terminação de Peptídeos
/
Biologia Celular
/
Proteínas de Saccharomyces cerevisiae
/
Genética
/
Metabolismo
Tipo de estudo:
Estudo prognóstico
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2012
Tipo de documento:
Artigo
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