Screening of food-grade microorganisms for biotransformation of D-tagatose and cloning and expression of L-arabinose isomerase / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 592-601, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-342458
ABSTRACT
L-Arabinose isomerase (L-AI) is an intracellular enzyme that catalyzes the reversible isomerization of D-galactose and D-tagatose. Given the widespread use of D-tagatose in the food industry, food-grade microorganisms and the derivation of L-AI for the production of D-tagatose is gaining increased attention. In the current study, food-grade strains from different foods that can convert D-galactose to D-tagatose were screened. According to physiological, biochemical, and 16S rDNA gene analyses, the selected strain was found to share 99% identity with Pediococcus pentosaceus, and was named as Pediococcus pentosaceus PC-5. The araA gene encoding L-AI from Pediococcus pentosaceus PC-5 was cloned and overexpressed in E. coli BL21. The yield of D-tagatose using D-galactose as the substrate catalyzed by the crude enzyme in the presence of Mn2+ was found to be 33% at 40 degrees C.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Pediococcus
/
Proteínas Recombinantes
/
Biotransformação
/
Classificação
/
Clonagem Molecular
/
Aldose-Cetose Isomerases
/
Escherichia coli
/
Galactose
/
Vetores Genéticos
/
Genética
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2012
Tipo de documento:
Artigo
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