Expression and molecular evolution of recombinant acetylcholinesterase for detection of pesticide residues: a review / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 557-564, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-342461
ABSTRACT
Acetylcholinesterase (AChE) plays a key role in the pesticide determination. However, the extraction of AChE from natural materials has the disadvantages of low yield, complex purification and poor stability. Therefore, the preparation of recombinant AChE with high performance becomes the hot topic of researchers in recent years. In this article we summarize the progress in the expression of recombinant AChE and the improvement of its analytical characteristic. Finally, we point out that the directed evolution strategy combined with surface display technology is the future trend on improving recombinant AChE activity.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Pichia
/
Acetilcolinesterase
/
Proteínas Recombinantes
/
Resíduos de Praguicidas
/
Química
/
Inibidores da Colinesterase
/
Baculoviridae
/
Evolução Molecular
/
Técnicas de Visualização da Superfície Celular
/
Vetores Genéticos
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2012
Tipo de documento:
Artigo
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