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Recent progress in fusion enzyme design and applications / 生物工程学报
Chinese Journal of Biotechnology ; (12): 393-409, 2012.
Artigo em Chinês | WPRIM | ID: wpr-342476
ABSTRACT
Engineering and redesign of enzymes are important to industrial biocatalysis. Fusion enzyme technology, based on fusion protein design, is frequently used in multifunctional enzyme construction and enzyme proximity control. Here, we reviewed the recent progress in molecular design strategy and application studies of fusion enzymes. The concept and features of fusion enzymes were introduced, followed by a systematical summary of the design strategy of fusion enzymes. In particular, the effects of different linker properties on fusion enzymes and their possible mechanisms were discussed. In addition, recent studies on fusion enzyme applications were also discussed. Finally, based on our own studies on fusion enzymes and the current research progress, the key problems in fusion enzyme technology and perspectives of this field were discussed.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Biotecnologia / Proteínas Recombinantes de Fusão / Engenharia de Proteínas / Química / Enzimas / Biocatálise Idioma: Chinês Revista: Chinese Journal of Biotechnology Ano de publicação: 2012 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Biotecnologia / Proteínas Recombinantes de Fusão / Engenharia de Proteínas / Química / Enzimas / Biocatálise Idioma: Chinês Revista: Chinese Journal of Biotechnology Ano de publicação: 2012 Tipo de documento: Artigo