Screening and identification of stable transfectants of mouse soluble B lymphocyte stimulator / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 897-900, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-342585
ABSTRACT
Mouse colon cancer cells CT26 were transfected with constructed plasmid expressing mouse soluble B lymphocyte stimulator (msBlyS). Single cell clones were selected with 100 microg/ml Zeosin and subcloned by serial limiting dilution. Eight resistant transfectants were isolated and expanded, and five of them displayed the desirable msBlyS cDNA band amplified by semi-quantitative RT-PCR assay. Western blot analysis showed that only msBlyS molecules of the expected size were detected in the cell lysates from transfectants. The supernatant of transfectants could costimulate B cell proliferation in standard costimulation assay. Thus we have successfully screened the stable transfectants expressing high levels of msBlyS in CT26 cells, which could be used as cancer vaccines for further anti-tumor immunotherapy.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Recombinação Genética
/
Proteínas Recombinantes
/
Células Tumorais Cultivadas
/
Linfócitos B
/
Transfecção
/
Fator de Necrose Tumoral alfa
/
Clonagem Molecular
/
Neoplasias do Colo
/
DNA Complementar
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
/
Estudo de rastreamento
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2004
Tipo de documento:
Artigo
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