Preparation of recombinant human insulin--study of downstream process / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 805-808, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-342607
ABSTRACT
This study was intended to establish a method of preparation of recombinant human insulin, with (His)6-Arg-Arg-human proinsulin (RRhPI) expressed by Escherichia coli. After DEAE-Sepharose Fast Flow ion-exchange chromatography, Sephadex G-25 chromatography and refolding, enzyme cleavage and Superdex 75 size exclusion chromatography,the RRhPI expressed by Escherichia coli in inclusion body form was converted to human insulin. The obtained recombinant human insulin was analyzed by SDS-PAGE, HPLC, amino acid composition analysis and bioidentity test (mouse convulsion test). The results indicate that our obtained preparation is highly purified, active recombinant human insulin.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proinsulina
/
Proteínas Recombinantes
/
Cromatografia Líquida de Alta Pressão
/
Escherichia coli
/
Genética
/
Insulina
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2004
Tipo de documento:
Artigo
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