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Preparation of recombinant human insulin--study of downstream process / 生物医学工程学杂志
Journal of Biomedical Engineering ; (6): 805-808, 2004.
Artigo em Chinês | WPRIM | ID: wpr-342607
ABSTRACT
This study was intended to establish a method of preparation of recombinant human insulin, with (His)6-Arg-Arg-human proinsulin (RRhPI) expressed by Escherichia coli. After DEAE-Sepharose Fast Flow ion-exchange chromatography, Sephadex G-25 chromatography and refolding, enzyme cleavage and Superdex 75 size exclusion chromatography,the RRhPI expressed by Escherichia coli in inclusion body form was converted to human insulin. The obtained recombinant human insulin was analyzed by SDS-PAGE, HPLC, amino acid composition analysis and bioidentity test (mouse convulsion test). The results indicate that our obtained preparation is highly purified, active recombinant human insulin.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Proinsulina / Proteínas Recombinantes / Cromatografia Líquida de Alta Pressão / Escherichia coli / Genética / Insulina / Metabolismo Limite: Humanos Idioma: Chinês Revista: Journal of Biomedical Engineering Ano de publicação: 2004 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Proinsulina / Proteínas Recombinantes / Cromatografia Líquida de Alta Pressão / Escherichia coli / Genética / Insulina / Metabolismo Limite: Humanos Idioma: Chinês Revista: Journal of Biomedical Engineering Ano de publicação: 2004 Tipo de documento: Artigo