Engineering and expression of sequence-specific DNA-binding zinc finger protein / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 662-667, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-342769
ABSTRACT
This experiment was aimed to create A20 gene site-specific zinc finger DNA-binding protein. The sequence of A20 gene promoter was analyzed with bioinformatics means and submitted to ZF Tools Server at TSRI. Using the database of the web site, we determined the A20 gene valid target sites and designed the amino acid sequence of zinc finger protein predicted to be bound to the target site. And then, the structure of the protein sequence was analyzed and homology was modeled with various bioinformatics means. Based on the characteristic of this protein, the prokaryotic expression vector pTYB11-ZFP was constructed and expressed. Thus, the artificial zinc finger protein that recognized A20 specific sequence was designed, and expressed in Escherichia coli. The results indicate that it is feasible to design engineered artificial Zinc finger proteins by means of bioinformatics.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Ligação Proteica
/
Fatores de Transcrição
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Engenharia de Proteínas
/
Química
/
Dedos de Zinco
/
Sequência de Aminoácidos
/
Proteínas de Ligação a DNA
/
Genética
Tipo de estudo:
Estudo prognóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2008
Tipo de documento:
Artigo
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