Construction and identification of rat GDNF gene recombinant retroviral vector and gene transfection to NSC / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 642-646, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-342773
ABSTRACT
By genetic recombinant technique, the rat GDNF cDNA was recombinated to the retroviral vector pLXSN. The recombinant plasmid pLXSN-GDNF was verified by digestion with restriction endonucleases and PCR. Then neural stem cells (NSCs) were infected with pLXSN-GDNF. Immunocytochemistry, RT-PCR and western-blot were used to detect the transfection effect. Results showed that GDNF cDNA was cloned into retroviral vector pLXSN correctly, and the pLXSN-GDNF can infect NSCs efficiently. These results provide the possibility for transplantation and gene therapy with GDNF of nervous system diseases and injury.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Células-Tronco
/
Proteínas Recombinantes
/
Terapia Genética
/
Adenoviridae
/
Clonagem Molecular
/
Técnicas de Transferência de Genes
/
DNA Complementar
/
Biologia Celular
/
Fator Neurotrófico Derivado de Linhagem de Célula Glial
/
Vetores Genéticos
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2008
Tipo de documento:
Artigo
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