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Enhanced production of curdlan by Alcaligenes faecalis by selective feeding with ammonia water during the cell growth phase of fermentation / 生物工程学报
Chinese Journal of Biotechnology ; (12): 1035-1039, 2008.
Artigo em Chinês | WPRIM | ID: wpr-342797
ABSTRACT
Curdlan is a water insoluble exopolysaccharide produced by Alcaligenes faecalis under nitrogen-limiting conditions. After excretion, the polysaccharide is attached the cell wall. Thus enhancement of biomass production during the cell growth phase is important to curdlan production. A strategy of increasing nitrogen source to improve biomass production was adopted for curdlan production by Alcaligenes faecalis (ATCC 31749). In the batch fermentation of curdlan, a relatively higher NH4Cl level of 3.6 g/L with continuous glucose feeding increased the cell density leading to improvement of curdlan production. However, excessive NH4Cl would inhibit curdlan production and biomass production was not improved significantly. In addition, feeding of ammonia water at the initial phase replaced NaOH solution to control pH at 7.0. Subsequently, feeding of NaOH solution was resumed to control pH at 5.6 for curdlan production after ammonia was consumed. As a result, biomass production and curdlan yield were both enhanced remarkably. Feeding of ammonia water during the first 24 h led to biomass production of 18.8 g/L. However, higher cell density did not lead to increase in curdlan production. The maximum curdlan production (72 g/L) was obtained by feeding ammonia water for the first 14 h, during which the cell density was about 11.9 g/L.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Técnicas de Cultura de Células / Biologia Celular / Alcaligenes faecalis / Beta-Glucanas / Proliferação de Células / Fermentação / Cloreto de Amônio / Metabolismo / Métodos Idioma: Chinês Revista: Chinese Journal of Biotechnology Ano de publicação: 2008 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Técnicas de Cultura de Células / Biologia Celular / Alcaligenes faecalis / Beta-Glucanas / Proliferação de Células / Fermentação / Cloreto de Amônio / Metabolismo / Métodos Idioma: Chinês Revista: Chinese Journal of Biotechnology Ano de publicação: 2008 Tipo de documento: Artigo