Construction and characterization of a novel somatostatin prokaryotic expression / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 995-998, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-342804
ABSTRACT
In the current work, the fusion gene including somatostatin (SS) and the hepatitis B surface antigen gene was cloned into a balanced lethal system plasmid (pYA3493), and then transformed into asd- attenuated Salmonella choleraesuis C500 strain, the positive transformant without antibiotic resistance gene was confirmed by restriction analysis and DNA sequencing, designated as pYA-SS. The expression and immunogenicity of fusion protein were detected by SDS-PAGE and Western blot analysis. These results show that the recombinant prokaryotic expression plasmid pYA-SS could express the SS fusion protein with good immunogenicity in C500 strain. In above all, this study could provide reliable materials to develop novel, good and safe vaccine in enhancing the growth of animals.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmídeos
/
Células Procarióticas
/
Salmonella arizonae
/
Proteínas Recombinantes de Fusão
/
Somatostatina
/
Clonagem Molecular
/
Fusão Gênica Artificial
/
Alergia e Imunologia
/
Genética
/
Antígenos de Superfície da Hepatite B
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2008
Tipo de documento:
Artigo
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